Probiotic Strain Level Viable Count Using Viability PCR

This application note presents the development and validation of a viability real-time PCR (v-PCR) method for strain-specific enumeration of Lacticaseibacillus rhamnosus GG in probiotic products. Fill out the form to learn more about how the v-PCR method offers high specificity, fast and accurate results, and the ability to quantify viable but non culturable (VBNC) cells, providing a valuable tool for quality control and compliance in probiotic manufacturing.

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Learnings

What You Can Expect

Explore valuable, science-backed insights designed to expand your knowledge and keep you informed.

Limitations of Plate Count and Benefits of v-PCR

v-PCR overcomes plate count limitations by detecting viable but non-culturable cells, improving accuracy in probiotic viability assessment.

Developing a Strain-Specific v-PCR for GG

A validated v-PCR method for L. rhamnosus GG uses optimized dye treatment and DNA release for accurate viability measurement.

Comparing v-PCR and Plate Count Performance

A validated v-PCR method for L. rhamnosus GG uses optimized dye treatment and DNA release for accurate viability measurement.

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